For nine years, two dozen genetic engineers struggled to create a
simple soybean that would stand up to a killer herbicide.
After tens of thousands of mistakes, they thought they might have done
it: They had created 100 seedlings laced with DNA from soil bacteria, a
cauliflower virus and a petunia plant. They planned to test them
cautiously in their Monsanto Co. labs. But an eager executive decided to
test them all, to douse every plant with a highly potent concentration of
The team leader, Stephen Padgette, raced to the greenhouse to plead
that some seedlings be spared. But he was too late; the plants were
Every one of them survived.
They would go on to become the first blockbuster biotech crop,
sweeping across America's farms and into America's diet with astounding
Genetically modified soy has been on the market just five years. Yet
it already accounts for two-thirds of the U.S. soybean harvest. Soy
products are used in hundreds of processed foods, often to add texture
and protein. So the biotech beans end up in pancake mix and baby formula,
chicken soup and margarine, crackers and salad dressing, ice cream and
From lab to field to table, the story of engineered soy offers a
window into the biotechnology revolution. It was the first staple crop to
be successfully engineered and widely planted. And it offers the
longest-running case study of the biotech experiment.
Five years in, there are signs that the rapid spread of transgenic
crops may be upending agricultural ecosystems--throwing colonies of soil
microbes out of balance and shifting the types of weeds that crop up most
often on fertile fields.
The experience of biotech soy also points up the lack of federal
regulation, especially compared with other countries. The soy appeared in
processed food even before the manufacturers knew it was there. And
though Monsanto conducted extensive safety tests, critics warn that they
were inadequate and raise questions about the enormous economic power
that a company such as Monsanto wields in this new world.
The United States has not seen the same type of anti-biotech protests
that have roiled Europe in recent years. But vandals protesting genetic
engineering have destroyed some labs and test fields in their fury.
And there is a small but fierce rebellion brewing among farmers who
contend that the biotech business model is strangling the heartland by
giving Monsanto too much power over global agriculture.
Though competitors such as DuPont Co. and Dow Chemical Co. are working
to catch up, Monsanto dominates the biotech market: Well over 90% of all
transgenic crops planted worldwide were developed at the firm's high-tech
labs here in suburban St. Louis. Monsanto's scientists have engineered
not only two-thirds of the soy crop, but also two-thirds of all the
cotton and a quarter of all the corn grown in the United States.
The reason for Monsanto's success is straightforward: The new seed is
easier and often cheaper to grow. It can reduce the need for chemicals to
control weeds and pests.
Transgenic soy, cotton and corn are now planted on more than 75
million acres in every state except Alaska, Hawaii, Nevada and Rhode
Island. Most U.S. livestock eat feed made with biotech grain. And 70% of
processed foods have biotech ingredients. Despite bitter European
protests, the crops are increasingly popular overseas as well, especially
in parts of Asia and South America.
Monsanto executives see genetic engineering as a wonder tool that can
help alleviate hunger by making food more nutritious and easier to grow.
They take their triumph with these first few crops as proof that they can
change the world. Their critics fear just that.
Biotech soy was born of a business brainstorm.
In the late 1970s, as today, Monsanto's leading product was a
herbicide called Roundup, which is made from glyphosate, an incredibly
effective chemical that kills almost everything green. Farmers worldwide
relied on Roundup to clear their fields before planting. But they could
not spray it once their seeds sprouted, because it would kill their crop
along with the weeds.
Monsanto set out to boost sales of Roundup by creating crops that
would tolerate glyphosate.
It would take 700,000 hours of work.
Padgette's team spent the first seven years on the wrong track
altogether, trying to rearrange soybean DNA by hand. "At a certain
point," Padgette recalled, "we decided we needed to think outside the
Instead of toying with soy's existing genes, they decided to try to
add new ones. Glyphosate works by binding to an enzyme in plants that
produces proteins critical for growth. With the glyphosate clinging to
it, the enzyme can't function and the plant dies. Bacteria have that same
critical enzyme. So in 1987, Padgette's group began screening bacteria to
see whether any had a natural resistance to glyphosate.
Two years later, with the help of a robot that ran analyses all night,
they found one that did. In this bacterium, the enzyme critical for
growth had a slightly different chemical structure, so glyphosate could
not bind to it. It was, Padgette said, "a great Eureka moment."
Not that he allowed time to celebrate. Working late nights, jazz music
blaring, the team turned to the hardest chore: figuring out how to make
the bacterial DNA perform its magic in soy.
The key turned out to be a gene packet containing DNA from four
A gene from a cauliflower virus acted as a master control switch. It
activated the bacterial enzyme that was able to fend off Roundup while
still producing adequate growth proteins. A snippet of petunia DNA made
sure those proteins were ferried to the proper location within the soy
plant. Another strand of DNA from a different type of bacterium served as
a molecular stop sign, preventing overproduction of the proteins.
Padgette's team bundled those four strands of DNA together. Then they
used a gene gun to plug the whole packet into individual soybean cells.
The process worked like this: DNA strands were wrapped around tiny gold
pellets, smaller than dust. The gene gun then fired the pellets at
soybean embryos. As the pellets ripped through the embryo, at velocities
of up to 1,400 feet per second, the DNA was wiped off, sticking in the
nuclei of individual cells.
Trouble was, the gene gun inserted the DNA at random. Sometimes a
bundle would splinter before landing in a cell. Or two gene packets would
double up. Even worse, the DNA would at times land in a spot that
interfered with cellular function. The team had to fire the gun tens of
thousands of times to get a few dozen plants that looked promising.
After three years of field tests on these promising plants, a single
line of transformed soybean shone as superior. It could resist heavy
doses of glyphosate, as the greenhouse experiment proved. And it looked
great in the field, matching conventional varieties bean-for-bean in
yield, growth and quality. "It was bulletproof," Padgette recalled with
pride. In 1993, Monsanto declared it a winner.
The embryo that had been labeled "40-3-2" during the gene-gun trials
had emerged as the first transgenic soybean. Monsanto called it "Roundup
Ready," because it could be sprayed with the herbicide and suffer no
harm. Just one hurdle remained before taking it to market.
Though the Food and Drug Administration did not require any safety
tests, Monsanto executives wanted to be sure they had a seal of approval
from that agency, as well as from the U.S. Department of Agriculture and
the Environmental Protection Agency.
But there was no regulatory process in place.
So Roy Fuchs, Monsanto's director of regulatory science, was called
upon to invent one. He came up with a list of tests he thought Monsanto
should run to prove the soybeans safe. Then he compared notes with
Although the FDA did not require any specific safety tests of biotech
food, other agencies around the world did. Monsanto plunged ahead.
Roundup Ready soybeans were fed to quail, cattle and rats, to catfish
in Mississippi and chickens in Missouri. There were toxicity tests to
make sure the bean wasn't dangerous even in huge quantities. There were
feed tests to make sure animals gained as much weight on a transgenic
The foreign protein in Roundup Ready soy was matched against a
computer database to make sure it did not resemble any known allergen. It
was plunked into simulated intestinal juice to see how the human gut
would react. Every nutritional component of the biotech bean was analyzed
and matched against conventional varieties. In all, Monsanto spent up to
$8 million on safety tests. The goal was to show a "reasonable certainty"
that the beans would do no harm--the FDA standard used for ordinary food.
"A lot of people who oppose biotech would like us held to a standard
of absolute safety, which is not possible," Fuchs said. "We would have to
reinvent food, because food is not inherently safe." (Indeed, food
produced through conventional cross breeding has at times proved
dangerous, from a potato loaded with toxins to a celery that causes a
Monsanto aimed to show that its transgenic crops were "substantially
equivalent" to conventional ones. The FDA and several international
groups, including the World Health Organization, have approved this
standard. Using it, Monsanto has won approval for its soy from 30
regulatory agencies in 18 countries. Opponents, however, are not
"You're producing combinations of genes that cannot be made in
nature," said Margaret Mellon, a molecular biologist and prominent foe of
biotechnology. "You're putting bits of DNA, in some cases whole proteins,
into crops when they've never been in the food system before. It seems to
me that level of novelty is enough to merit an extra degree of scrutiny."
"It's difficult to say anything is 100% certain, but we have a good
handle on what we ought to be looking at" to ensure food safety,
responded Laura Tarantino, the FDA's deputy director of premarket
approval. "We're trying to use the best set of tests we know."
The FDA has proposed making safety testing mandatory for biotech food.
But the agency has no plans to dictate which tests companies should
perform. "We're not explicit," Tarantino said, "but we expect them to
come in early in the process to consult with us."
To critics, letting biotech companies define and measure safety is
akin to letting a student write and grade his own exam.
Even Dan Glickman, former Agriculture secretary, has expressed concern
that products such as Roundup Ready beans have not received enough
objective scrutiny, though he says he still believes they are safe.
Glickman supervised approval of several biotech products that followed
Monsanto's soy. Looking back now, he regrets that industry was allowed to
take the lead, as regulators largely ceded their watchdog role.
"Regulators even viewed themselves as cheerleaders for biotechnology," he
said. "It was viewed as science marching forward, and anyone who wasn't
marching forward was a Luddite."
In 1996, their first year on the market, Roundup Ready beans were
planted on 1 million acres. The next year, that figure was up to 9
million. The year after, 27 million. This year, the USDA projects biotech
soybeans will be sown on 48 million acres. Monsanto estimates 90% of soy
growers have tried them, though some have rejected them as not
cost-effective for their particular fields.
The transgenic soy (and, later, corn and cottonseed oil) entered the
food stream so quickly that most processors did not even realize they
were using it. It was not until Europeans began protesting "Frankenfoods"
that U.S. makers of processed food began to investigate the safety of
ingredients they had been using for at least two years.
"We really learned after the fact, after these ingredients were
already in the food supply," said Lisa Katic, director of scientific
policy for the Grocery Manufacturers of America.
After a year of intense review, Katic said her group's members are
convinced the biotech crops on the market today are safe to eat. And,
indeed, no serious scientist has suggested otherwise.
Instead, five years after the introduction of Roundup Ready soy, many
critics are turning their attention to the beans' performance in the
field--and to possible environmental effects.
Monsanto says its research shows its beans perform every bit as well
as conventional varieties. They also have some positive environmental
spinoffs. Farmers do not have to churn the soil as much to loosen weeds,
which cuts down on erosion. And because glyphosate is so effective,
soybean growers have slashed both the number of chemicals they spray and
the number of fuel-burning trips they take across their fields.
But there have been some worrisome indicators too.
Research from the University of Georgia suggests that the genetic
changes may have affected the soybean metabolism, causing stalks to grow
brittle and split in extreme heat. A study at the University of Arkansas
found some biotech beans fared badly in drought, perhaps because the
Roundup spray damaged the soil bacteria that help soy plants draw in
Perhaps most troubling, USDA soil scientist Robert Kremer found in a
four-year study that spraying Roundup over biotech beans seemed to touch
off wild proliferation of fungi in the soil. Some fungi are fatal to
soybeans. But Kremer says it's not short-term crop damage that concerns
him. It's long-term shifts in soil ecology if Roundup is sprayed season
after season on our most fertile fields.
"These microbes in the soil are in a natural balance with each other,
and if we keep putting the same chemicals in year after year, that could
lead to a shift in the soil ecosystem, which could lead to problems down
the road in terms of the soil's ability to nurture plant life," Kremer
said. Monsanto says it is still studying the issue.
Of equal concern are the shifts some farmers have noticed in weed
Since the introduction of Roundup Ready crops, glyphosate use has
soared. It was applied on 20% of U.S. farm acreage in 1995; four years
later, on 62%. (Though there are competing brands from other companies,
the boom has been a windfall for Monsanto: Roundup sales brought in $2.6
billion last year.) Some farmers now plant Roundup Ready crops
year-round, rotating corn and soybeans; they may apply glyphosate four to
six times a year on a single field.
Though the EPA has determined that spraying crops with Roundup has no
ill effect on the consumer, critics worry that such heavy use will speed
the emergence of weeds that can tolerate glyphosate--and will force
farmers and home gardeners alike to turn to more toxic herbicides.
Some farmers are starting to see morning glory vine and yellow nut
sedge, the weeds Roundup has always had the toughest time controlling,
moving aggressively into their fields. Some need to mix other herbicides
with their glyphosate for total weed control, said Don Schafer, a soybean
product manager at Pioneer Hi-Bred International, a major seed company.
"By the time the industry acknowledges a problem, the
glyphosate-resistant genes will have spread far enough through the weed
population that not much can be done," warns Charles Benbrook, a biotech
critic who directs the Northwest Science and Environmental Policy Center
in Sandpoint, Idaho. "This is not the kind of thing where you can put the
genie back in the bottle."
Monsanto executives point out that Roundup has been on the market for
25 years and there are only two confirmed examples of weeds that can
withstand it: one in Australia and one in Malaysia. (They are studying
two other recent reports of tolerant weeds in Delaware and Missouri,
however.) What's more, they say, Roundup has long been applied four to
six times a year on tropical crops such as Florida citrus, without
sparking widespread weed shifts.
Many growers, however, seem resigned that weeds will one day outsmart
Roundup. "It's happened with every herbicide we have," said Don Latham,
an Iowa farmer.
That conviction doesn't stop him from using Roundup Ready beans,
though. In fact, Latham loves them. With conventional soy, he makes two
passes over each field to spray three different herbicides--and still
spends hours pulling stray weeds by hand. With the biotech crop, he
sprays glyphosate once and kills every weed. Even factoring in the higher
price of Roundup beans, he saves $22.50 an acre on chemicals and fuel. To
him, biotech is a bargain.
Other growers are not so sure.
When a farmer buys a 50-pound sack of Roundup Ready soybeans, enough
to plant about an acre and a quarter, he pays an extra $6.50 "technology
fee." He also has to sign a two-page contract promising not to save beans
from his harvest to plant as seed the following season. This is a radical
demand. Traditionally, about a third of U.S. soybean growers have saved
some of their harvest for seed, which saves them money.
Monsanto's rationale is clear. It spent at least $80 million to
develop biotech soy. It owns eight patents on Roundup Ready beans, plus
another two dozen on technologies developed during the 13 years of
research and testing it took to bring the soy to market. Now, it's
payback time. Or, as Brett Begemann, a vice president, put it: "To make
that kind of investment, you have to get a return."
The company operates a toll-free hotline to collect tips about farmers
who might be saving seed illegally. And tipsters call by the hundreds,
eager to blow the whistle on competitors getting the Roundup gene for
free. Monsanto's investigators--the "bean police" to angry
farmers--follow up on every tip. Quite often, Begemann said, the cases
are resolved with the offending grower agreeing to pay a fine. But
Monsanto also has taken scores to court.
Among the pending cases is a suit against Mitchell Scruggs, a
51-year-old soy and cotton farmer near Tupelo, Miss.
Scruggs likes the Roundup trait. But he hates Monsanto's contract. In
open defiance, he saved and replanted his biotech seed four years
running. Then the company sued him. He has countersued with an antitrust
claim, arguing that Monsanto is colluding with seed companies and
retailers--who get a cut of the technology fee--to squeeze farmers dry.
"They're trying to control all the food and fiber in the world by
monopolizing the seed industry," Scruggs said, an edge to his Southern
Hoping to spark a farmers' rebellion, Scruggs recruited 200 neighbors
not long ago and founded the "Save Our Seed" movement. They advertised
their wrath in farm publications and received, Scruggs says, some 18,000
calls from irate farmers nationwide. Scruggs' attorney now has antitrust
cases against Monsanto in two other states.
Monsanto also is catching flak from critics over its proprietary
attitude toward the genes and technology that made its biotech
blockbusters possible and power its $5.5 billion in annual sales.
Monsanto does license its technology widely so other researchers can
use its techniques. It also donates its know-how to work on Third World
staples such as cassava. But that track record does not reassure critics
who warn that Monsanto and a few other biotech leaders have increasing
power to choke off competing research.
"Who will determine the future of agricultural research? Are we as a
society willing to turn this over to a handful of companies?" asked Hope
Shand, research director of the Rural Advancement Foundation
Monsanto, of course, stands by its business model. And it stands by
Roundup Ready. Among the dozens of biotech crops in its pipeline: Roundup
Ready wheat, rice, alfalfa and sugar beets.
Those who fueled this revolution, who spent nights analyzing soil
bacteria in the lab and days juggling test tubes with a robot, think of
Roundup Ready as a marvel. It has been a profit machine for Monsanto, of
course. But they say it has transformed farming as well. They are proud,
and still slightly amazed, that they pulled it off.
Padgette tried recently to put the feeling into words. This was the
best he could do: "You just sit back and you say, 'Wow.' "
Copyright © 2001 Los Angeles Times